Publication Title

Acute Toxicity Evaluation Of Moringa Oleifera Leaves Extracts Of Ethanol And Water In Mice.
Authors: 
J.N. Kasolo1*,, G.S. Bimenya2,, A. L. Okwi2,, E.M. Othieno2,, J.W. Ogwal-Okeng3
Volume: 
7
Year of Publication: 
2012

Publication Abstract

The leaves of Moringa oleifera contain a profile of important trace elements such as phytochemicals, proteins, vitamins, beta-carotin and amino acids. While the plant is consumed by many families worldwide due to its attributes, its toxicity profile is not well documented. A laboratory based study was therefore carried out to identify phytochemicals in the leaves of Moringa oleifera and establish their acute toxicity or lethal dose (LD50) profile in mice. Leaves of this plant were harvested during dry season, air dried and pounded. Serial extraction of the phytochemicals was done using ether, ethanol and water as solvents. The phytochemicals were qualitatively identified. Different doses of ethanol and aqueous extracts were given in a single oral dose to 5 male and 5 female Swiss Albino mice, aged 6-8 weeks. The number of animals that died in 24 hours was recorded and their behavioral change was noted during that period. The lethal dose (LD50) of each of these extracts was calculated using probit tables. Gallic tannins, catechol tannins, steroids, triterponoids, flavonoids, saponins, anthraquinones, alkaloids and reducing sugars were extracted. The LD50 of Moringa oleifera leaves ethanol extract given intragastric was found to be 39.6 g/kg body weight while that of the aqueous extract was 16.1 g/kg. In conclusion it was shown that Moringa oleifera leaves contained phytochemicals were not toxic when given in a single oral dose in 24 hours.