Publication Title

Antipyretic And Analgesics Activities Of Ethanolic Extract Of Pasea Americana Mill Seeds In Wistar Albino Rats
Authors: 
Al. H. Kyakulaga11,2*., P.Engeu Ogwang, S. Nannyonga 2., J. Nyafuono 3 , R. Tumusiime 3
Volume: 
7
Year of Publication: 
2012

Publication Abstract

The ethanolic extract of Persea Americana Mill seed was tested for both antipyretic and analgesic activities using Wistar albino rats. The seeds were dried for 14 days under the shed. Dry powdered P. americana seeds (500g) was extracted using 90% ethanol solvent system by cold maceration for 72 hours, filtered and concentrated using a rotary evaporator. Saccharomyces cerevisiae (suspended in pyrogen-free 0.9% NaCl in a water bath at 37 °C for 5 minutes) was used to induce fever in 40 Wistar rats. Animals with rectal temperatures (36°C -37°C) were randomly selected and grouped into four groups (n=8). Two of the groups received plant extracts (200mg/Kg or 400mg/Kg), one received 100mg/kg paracetamol while fourth group received distilled water. Rectal temperatures were then monitored hourly over an 8 hour period. Experimental pain (writhing) was induced in Swiss albino mice by intraperitoneal injection of 10 ml/kg of 0.6% acetic acid. The number of writhing was counted over a 20 minute period. Preliminary phytochemical analysis revealed prescence of flavonoids, coumarins and steroid glycosides. P. Americana Mill ethanol extract at dose level of 400mg/kg was as effective and rapid in controlling fever as 100mg/Kg of paracetamol (table below). The extract also caused significant analgesia at dose level of 400mg/Kg which was comparable to 100mg/Kg of Aspirin (p=0.541). In conclusion Ethanolic extract of P. Americana had both analgesic and antipyretic activity. Further fractionation and purification of this extract could yield potent compounds for therapeutic application in the management of fevers and pain.